Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall architecture in <i>Brachypodium distachyon</i>
Name
New Phytologist - 2024 - Tryfona - Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall.pdf
Description
Published version
Size
2.5 MB
Format
Adobe PDF
Checksum (MD5)
d2d121826711304c3bf3b4b9e0b527cd
Author(s)
Tryfona, Theodora
Pankratova, Yanina
Petrik, Deborah
Rebaque Moran, Diego
Wightman, Raymond
Yu, Xiaolan
Echevarría‐Poza, Alberto
Deralia, Parveen Kumar
Vilaplana, Francisco
Anderson, Charles T
Date Issued
February 27, 2024
Journal
New Phytologist
Publisher
Wiley
Citation
Tryfona, T., Pankratova, Y., Petrik, D., Rebaque Moran, D., Wightman, R., Yu, X., Echevarría-Poza, A., Deralia, P.K., Vilaplana, F., Anderson, C.T., Hong, M. and Dupree, P. (2024), Altering the substitution and cross-linking of glucuronoarabinoxylans affects cell wall architecture in Brachypodium distachyon. New Phytol, 242: 524-543.
Version
Final published version
Abstract
The Poaceae family of plants provides cereal crops that are critical for human and animal nutrition, and also, they are an important source of biomass. Interacting plant cell wall components give rise to recalcitrance to digestion; thus, understanding the wall molecular architecture is important to improve biomass properties. Xylan is the main hemicellulose in grass cell walls. Recently, we reported structural variation in grass xylans, suggesting functional specialisation and distinct interactions with cellulose and lignin. Here, we investigated the functions of these xylans by perturbing the biosynthesis of specific xylan types.
We generated CRISPR/Cas9 knockout mutants in Brachypodium distachyon XAX1 and GUX2 genes involved in xylan substitution. Using carbohydrate gel electrophoresis, we identified biochemical changes in different xylan types. Saccharification, cryo-SEM, subcritical water extraction and ssNMR were used to study wall architecture.
BdXAX1A and BdGUX2 enzymes modify different types of grass xylan. Brachypodium mutant walls are likely more porous, suggesting the xylan substitutions directed by both BdXAX1A and GUX2 enzymes influence xylan-xylan and/or xylan–lignin interactions.
Since xylan substitutions influence wall architecture and digestibility, our findings open new avenues to improve cereals for food and to use grass biomass for feed and the production of bioenergy and biomaterials.
Terms of Use
Creative Commons Attribution-NonCommercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1111/nph.19624
