Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
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Bhatia_Real-time imaging.pdf
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2.62 MB
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Author(s)
Jones, Christopher T.
Catanese, Maria Teresa
Law, Lok Man J.
Khetani, Salman R.
Syder, Andrew J.
Ploss, Alexander
Oh, Thomas S.
Schoggins, John W.
MacDonald, Margaret R.
Bhatia, Sangeeta N.
Date Issued
January 2010
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Jones, Christopher T et al. “Real-time Imaging of Hepatitis C Virus Infection Using a Fluorescent Cell-based Reporter System.” Nature Biotechnology 28.2 (2010): 167–171.
Version
Author's final manuscript
Abstract
Hepatitis C virus (HCV), which infects 2–3% of the world population, is a causative agent of chronic hepatitis and the leading indication for liver transplantation1. The ability to propagate HCV in cell culture (HCVcc) is a relatively recent breakthrough and a key tool in the quest for specific antiviral therapeutics. Monitoring HCV infection in culture generally involves bulk population assays, use of genetically modified viruses and/or terminal processing of potentially precious samples. Here we develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response, as well as visualizing infection of primary hepatocyte cultures. Integration of this reporter with modern image-based analysis methods could open new doors for HCV research.
Description
Author Manuscript 2010 August 1
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nbt.1604
