Erythrocyte structure and dynamics quantified by Hilbert phase microscopy
Name
Popescu-2005-Erythrocyte structur.pdf
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Author(s)
Popescu, Gabriel
Ikeda, Takahiro
Best, Catherine A.
Badizadegan, Kamran
Feld, Michael S.
Dasari, Ramachandra Rao
Date Issued
December 2005
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Popescu, Gabriel, Takahiro Ikeda, Catherine A. Best, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld. “Erythrocyte Structure and Dynamics Quantified by Hilbert Phase Microscopy.” Journal of Biomedical Optics 10, no. 6 (2005): 060503. © 2005 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
https://doi.org/10.1117/1.2149847
