Current Conjugation Methods for Immunosensors
Name
nanomaterials-08-00278.pdf
Size
16.16 MB
Format
Adobe PDF
Checksum (MD5)
916749294373d0689452088a14ed006c
Author(s)
Li, Zeyang
Chen, Guan-Yu
Date Issued
April 2018
Journal
Nanomaterials
Publisher
MDPI AG
Citation
Li, Zeyang and Guan-Yu Chen. "Current Conjugation Methods for Immunosensors." Nanomaterials 8, 5 (April 2018): 278 © 2018 The Authors
Version
Final published version
Abstract
Recent advances in the development of immunosensors using polymeric nanomaterials and nanoparticles have enabled a wide range of new functions and applications in diagnostic and prognostic research. One fundamental challenge that all immunosensors must overcome is to provide the specificity of target molecular recognition by immobilizing antibodies, antibody fragments, and/or other peptides or oligonucleotide molecules that are capable of antigen recognition on a compact device surface. This review presents progress in the application of immobilization strategies including the classical adsorption process, affinity attachment, random cross-linking and specific covalent linking. The choice of immobilization methods and its impact on biosensor performance in terms of capture molecule loading, orientation, stability and capture efficiency are also discussed in this review. Keywords: immunosensors; polymeric nanomaterials; immobilization methods
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/nano8050278
