CRISPR transcriptional repression devices and layered circuits in mammalian cells
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Weiss_Crispr transcriptional.pdf
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Author(s)
Kiani, Samira
Beal, Jacob
Huh, Jin
Xie, Zhen
Li, Yinqing
Weiss, Ron
Ebrahimkhani, Mohammad Reza
Hall, Richard N.
Date Issued
May 2014
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Kiani, Samira, Jacob Beal, Mohammad R Ebrahimkhani, Jin Huh, Richard N Hall, Zhen Xie, Yinqing Li, and Ron Weiss. “CRISPR Transcriptional Repression Devices and Layered Circuits in Mammalian Cells.” Nat Meth 11, no. 7 (May 5, 2014): 723–726.
Version
Author's final manuscript
Abstract
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Synthetic Biology Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.2969
