Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination
Name
16473.full.pdf
Description
Published version
Size
1.08 MB
Format
Adobe PDF
Checksum (MD5)
47843dc9c2b73419e4af58308bad9daf
Author(s)
Boopathy, Archana V
Mandal, Anasuya
Kulp, Daniel W.
Menis, Sergey
Bennett, Nitasha R
Watkins, Hannah C.
Wang, Wade
Martin, Jacob T
Thai, Nikki Tessa
He, Yanpu
Date Issued
July 2019
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Citation
Boopathy, Archana V. et al. "Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination." Proceedings of the National Academy of Sciences 116, 33 (July 2019): 16473-16478.
Version
Final published version
Abstract
Sustained exposure of lymphoid tissues to vaccine antigens promotes humoral immunity, but traditional bolus immunizations lead to rapid antigen clearance. We describe a technology to tailor vaccine kinetics in a needle-free platform translatable to human immunization. Solid pyramidal microneedle (MN) arrays were fabricated with silk fibroin protein tips encapsulating a stabilized HIV envelope trimer immunogen and adjuvant, supported on a dissolving polymer base. Upon brief skin application, vaccine-loaded silk tips are implanted in the epidermis/upper dermis where they release vaccine over a time period determined by the crystallinity of the silk matrix. Following MN immunization in mice, Env trimer was released over 2 wk in the skin, correlating with increased germinal center (GC) B cell responses, a ∼1,300-fold increase in serum IgG titers and a 16-fold increase in bone marrow (BM) plasma cells compared with bolus immunization. Thus, implantable MNs provide a practical means to substantially enhance humoral immunity to subunit vaccines.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1902179116
