A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays
Name
nihms-1508358.pdf
Description
Accepted version
Size
904.98 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Tam, Justina O.
de Puig Guixe, Helena
Yen, Chun-wan
Bosch, Irene
Gomez-Marquez, Jose Zacapa
Clavet, Charles
Hamad-Schifferli, Kimberly
Gehrke, Lee
Date Issued
January 2017
Journal
Journal of Immunoassay and Immunochemistry
Publisher
Informa UK Limited
Citation
Tam, Justina O. et al. "A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays." Journal of Immunoassay and Immunochemistry 38, 4 (January 2017): 355-377 © 2017 Taylor & Francis
Version
Author's final manuscript
Abstract
Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
SUTD-MIT International Design Centre (IDC)
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1080/15321819.2016.1269338
