DNA-CNT Nanowire Networks for DNA Detection
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Swager_DNA-CNT Nanowire.pdf
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560.38 KB
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Author(s)
Weizmann, Yossi
Chenoweth, David M.
Swager, Timothy M
Date Issued
February 2011
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Citation
Weizmann, Yossi, David M. Chenoweth, and Timothy M. Swager. “DNA−CNT Nanowire Networks for DNA Detection.” Journal of the American Chemical Society 133.10 (2011): 3238–3241.
Version
Author's final manuscript
Abstract
The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simple and effective conductometric devices. Here, we report a conductivity-based DNA detection method utilizing carbon nanotube−DNA nanowire devices and oligonucleotide-functionalized enzyme probes. Key to our sensor design is a DNA-linked-CNT wire motif, which forms a network of interrupted carbon nanotube wires connecting two electrodes. Sensing occurs at the DNA junctions linking CNTs, followed by amplification using enzymatic metalization leading to a conductimetric response. The DNA analyte detection limit is 10 fM with the ability to discriminate single, double, and triple base pair mismatches. DNA−CNT nanowires and device sensing gaps were characterized by scanning electron microscopy (SEM) and confocal Raman microscopy, supporting the enhanced conductometric response resulting from nanowire metallization.
MIT Department
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Chemistry
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ja109180d
