Probiotic strains detect and suppress cholera in mice
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eaao2586.full.pdf
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Author(s)
Mao, Ning
Cubillos, Andres Fernando
Cameron, Douglas
Collins, James J.
Date Issued
May 2018
Journal
Science Translational Medicine
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Mao, Ning et al “Probiotic Strains Detect and Suppress Cholera in Mice.” Science Translational Medicine 10, 445 (June 2018): eaao2586 © 2018 The Authors
Version
Final published version
Abstract
Microbiota-modulating interventions are an emerging strategy to promote gastrointestinal homeostasis. Yet, their use in the detection, prevention, and treatment of acute infections remains underexplored. We report the basis of a probiotic-based strategy to promote colonization resistance and point-of-need diagnosis of cholera, an acute diarrheal disease caused by the pathogen Vibrio cholerae. Oral administration of Lactococcus lactis, a common dietary fermentative bacterium, reduced intestinal V. cholerae burden and improved survival in infected infant mice through the production of lactic acid. Furthermore, we engineered an L. lactis strain that specifically detects quorum-sensing signals of V. cholerae in the gut and triggers expression of an enzymatic reporter that is readily detected in fecal samples. We postulate that preventive dietary interventions with fermented foods containing natural and engineered L. lactis strains may hinder cholera progression and improve disease surveillance in populations at risk of cholera outbreaks.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/scitranslmed.aao2586
