Transport of biomolecules in asymmetric nanofilter arrays
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Author(s)
Liu, G. R.
Li, Zi Rui
Hadjiconstantinou, Nicolas
Chen, Yu Zong
Wang, Jian-Sheng
Han, Jongyoon
Date Issued
January 2009
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer Berlin Heidelberg
Citation
Li, Zi, G. R. Liu, Jongyoon Han, Yu Zong Chen, Jian-Sheng Wang, and Nicholas G. Hadjiconstantinou. “Transport of biomolecules in asymmetric nanofilter arrays.” Analytical and Bioanalytical Chemistry 394.2 (2009): 427-435.
Version
Author's final manuscript
Abstract
We propose a theoretical model for describing the electric-field-driven migration of rod-like biomolecules in nanofilters comprising a periodic array of shallow passages connecting deep wells. The electrophoretic migration of the biomolecules is modeled as transport of point-sized Brownian particles, with the orientational degree of freedom captured by an entropy term. Using appropriate projections, the formulation dimensionality is reduced to one physical dimension, requiring minimal computation and making it ideal for device design and optimization. Our formulation is used to assess the effect of slanted well walls on the energy landscape and resulting molecule mobility. Using this approach, we show that asymmetry in the well shape, such as a well with one slanted and one vertical wall, may be used for separation using low-frequency alternating-current fields because the mobility of a biomolecule is different in the two directions of travel. Our results show that, compared to methods using direct-current fields, the proposed method remains effective at higher field strengths and can achieve comparable separation using a significantly shorter device.
Subjects
nanofluidics
asymmetric nanofilter
DNA separation
entropy barrier
Brownian ratchet
molecular transport
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1007/s00216-008-2558-y
