Embryonic stem cell-based mapping of developmental transcriptional programs
Name
Gifford-Embryonic stem cell.pdf
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1.73 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Mazzoni, Esteban O.
Mahony, Shaun
Iacovino, Michelina
Morrison, Carolyn A.
Mountoufaris, George
Closser, Michael
Whyte, Warren Anthony
Young, Richard A.
Kyba, Michael
Wichterle, Hynek
Date Issued
November 2011
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Mazzoni, Esteban O et al. “Embryonic Stem Cell–based Mapping of Developmental Transcriptional Programs.” Nature Methods 8.12 (2011): 1056–1058.
Version
Author's final manuscript
Abstract
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Whitehead Institute for Biomedical Research
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nmeth.1775
