3D RNA-scaffolded wireframe origami
Name
s41467-023-36156-1.pdf
Description
Published version
Size
3.4 MB
Format
Adobe PDF
Checksum (MD5)
4baff153573d6e09ddcfce3ac8f90605
Author(s)
Parsons, Molly F
Allan, Matthew F
Li, Shanshan
Shepherd, Tyson R
Ratanalert, Sakul
Zhang, Kaiming
Pullen, Krista M
Chiu, Wah
Rouskin, Silvi
Bathe, Mark
Date Issued
January 24, 2023
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Parsons, Molly F, Allan, Matthew F, Li, Shanshan, Shepherd, Tyson R, Ratanalert, Sakul et al. 2023. "3D RNA-scaffolded wireframe origami." Nature Communications, 14 (1).
Version
Final published version
Abstract
AbstractHybrid RNA:DNA origami, in which a long RNA scaffold strand folds into a target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to a limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization of long RNAs, and fabrication of artificial ribozymes. Here, we investigate design principles of three-dimensional wireframe RNA-scaffolded origami rendered as polyhedra composed of dual-duplex edges. We computationally design, fabricate, and characterize tetrahedra folded from an EGFP-encoding messenger RNA and de Bruijn sequences, an octahedron folded with M13 transcript RNA, and an octahedron and pentagonal bipyramids folded with 23S ribosomal RNA, demonstrating the ability to make diverse polyhedral shapes with distinct structural and functional RNA scaffolds. We characterize secondary and tertiary structures using dimethyl sulfate mutational profiling and cryo-electron microscopy, revealing insight into both global and local, base-level structures of origami. Our top-down sequence design strategy enables the use of long RNAs as functional scaffolds for complex wireframe origami.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-023-36156-1
