Antibody-Antigen assay design for combined optical tweezers and fluorescence
Name
57583131-MIT.pdf
Description
Full printable version
Size
1.12 MB
Format
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Checksum (MD5)
7ea151405670440540e4a0ec4464db43
Author(s)
Ta, Jenny, 1982-
Advisor(s)
Matthew J. Lang.
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
The recent development in combined optical trapping and fluorescence technology promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many biological systems. This thesis focuses on the development of assay designs for the study of antibody-antigen binding interactions using combined optical trapping and single molecule fluorescence. The assays create the necessary linkage geometry between the antibody-antigen system under study to an optically trapped bead, enabling force probing of the antibody-antigen binding interaction. In particular, two tether materials and fluorophores were studied: polyethylene glycol (PEG) with Cy-2, and dsDNA with fluorescein. We demonstrate tether formation in the dsDNA-fluorescein antibody-antigen linkage system with preliminary optical trapping data.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references (p. 37).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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