The sound of silence: Transgene silencing in mammalian cell engineering
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1-s2.0-S2405471222004641-main.pdf
Description
Published version
Size
3.62 MB
Format
Adobe PDF
Checksum (MD5)
bc0173e83c6b6bf7fdcf1c8dc957373f
Author(s)
Cabrera, Alan
Edelstein, Hailey I
Glykofrydis, Fokion
Love, Kasey S
Palacios, Sebastian
Tycko, Josh
Zhang, Meng
Lensch, Sarah
Shields, Cara E
Livingston, Mark
Date Issued
December 2022
Journal
Cell Systems
Publisher
Elsevier BV
Citation
Cabrera, Alan, Edelstein, Hailey I, Glykofrydis, Fokion, Love, Kasey S, Palacios, Sebastian et al. 2022. "The sound of silence: Transgene silencing in mammalian cell engineering." Cell Systems, 13 (12).
Version
Final published version
Abstract
To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to control the expression of all constituent transgene components. Transgene silencing, defined as the loss of expression over time, persists as an obstacle for engineering primary cells and stem cells with transgenic cargos. In this review, we highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgene silencing. We conclude with a perspective identifying future research directions for improving the performance of synthetic gene circuits.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.cels.2022.11.005
