Dopamine Neuron-Specific Optogenetic Stimulation in Rhesus Macaques
Name
1-s2.0-S0092867416310753-main.pdf
Description
Published version
Size
2.68 MB
Format
Adobe PDF
Checksum (MD5)
45a7bca77c8850e00652b1071f941f86
Author(s)
Stauffer, William R.
Lak, Armin
Yang, Aimei
Borel, Melodie
Paulsen, Ole
Boyden, Edward S.
Schultz, Wolfram
Date Issued
September 2016
Journal
Cell
Publisher
Elsevier BV
Citation
Stauffer, William R. et al. "Dopamine Neuron-Specific Optogenetic Stimulation in Rhesus Macaques." Cell 166, 6 (September 2016): P1564-1571.e6 © 2016 The Authors
Version
Final published version
Abstract
Optogenetic studies in mice have revealed new relationships between well-defined neurons and brain functions. However, there are currently no means to achieve the same cell-type specificity in monkeys, which possess an expanded behavioral repertoire and closer anatomical homology to humans. Here, we present a resource for cell-type-specific channelrhodopsin expression in Rhesus monkeys and apply this technique to modulate dopamine activity and monkey choice behavior. These data show that two viral vectors label dopamine neurons with greater than 95% specificity. Infected neurons were activated by light pulses, indicating functional expression. The addition of optical stimulation to reward outcomes promoted the learning of reward-predicting stimuli at the neuronal and behavioral level. Together, these results demonstrate the feasibility of effective and selective stimulation of dopamine neurons in non-human primates and a resource that could be applied to other cell types in the monkey brain.
MIT Department
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.cell.2016.08.024
