Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device
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Jensen_Nonendocytic delivery.pdf
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Author(s)
Lee, Jungmin
Sharei, Armon Reza
Sim, Woo Young
Adamo, Andrea
Langer, Robert S
Jensen, Klavs F
Bawendi, Moungi G
Date Issued
December 2012
Journal
Nano Letters
Publisher
American Chemical Society (ACS)
Citation
Lee, Jungmin, Armon Sharei, Woo Young Sim, Andrea Adamo, Robert Langer, Klavs F. Jensen, and Moungi G. Bawendi. “Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device.” Nano Lett. 12, no. 12 (December 12, 2012): 6322–6327.
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Author's final manuscript
Abstract
The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to probe the cell. In particular, quantum dots (QDs) may be used to illuminate cytosolic proteins for long-term microscopy studies. Whereas recent advances have been successful in specifically labeling proteins with QDs on the cell membrane, cytosolic delivery of QDs into live cells has remained challenging. In this report, we demonstrate high throughput delivery of QDs into live cell cytoplasm using an uncomplicated microfluidic device while maintaining cell viabilities of 80–90%. We verify that the nanoparticle surface interacts with the cytosolic environment and that the QDs remain nonaggregated so that single QDs can be observed.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/nl303421h
