Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
Name
Khoo-2016-Liquid biopsy and th.pdf
Size
1.41 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Khoo, B. L.
Grenci, G.
Jing, T.
Lim, Y. B.
Lee, S. C.
Thiery, J. P.
Lim, C. T.
Han, Jongyoon
Date Issued
July 2016
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Khoo, B. L. et al. “Liquid Biopsy and Therapeutic Response: Circulating Tumor Cell Cultures for Evaluation of Anticancer Treatment.” Science Advances 2.7 (2016): e1600274–e1600274.
Version
Final published version
Abstract
The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom microfabricated tapered microwells were integrated with microfluidics to allow robust formation of CTC clusters without pre-enrichment and subsequent drug screening in situ. Rapid feedback after 2 weeks promotes immediate intervention upon detection of drug resistance or tolerance. The procedure was clinically validated with blood samples (n = 73) from 55 patients with early-stage, newly diagnosed, locally advanced, or refractory metastatic breast cancer. Twenty-four of these samples were used for drug evaluation. Cluster formation potential correlated inversely with increased drug concentration and therapeutic treatment. This new and robust liquid biopsy technique can potentially evaluate patient prognosis with CTC clusters during treatment and provide a noninvasive and inexpensive assessment that can guide drug discovery development or therapeutic choices for personalized treatment.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-NonCommercial 4.0 International
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DOI of Published Version
https://doi.org/10.1126/sciadv.1600274
