Studies of the division cycle of human cancer cells (HELA) using a new system for continuous synchronization
Name
1371248901-MIT.pdf
Size
37.79 MB
Format
Adobe PDF
Checksum (MD5)
20eb8f94bb757fce6f25a5acc87aa1fa
Author(s)
Thilly, William George.
Advisor(s)
Gerald N. Wogan.
Date Issued
1971
Publisher
Massachusetts Institute of Technology
Abstract
HeLa S3 cells have been induced to divide synchronously in suspension culture through more than ten generations. Cellular DNA and RNA have been determined at hourly intervals throughout the cell cycle defining the pattern of nucleic acid accumulation in HeLa cells. Cellular activity of the enzymes thymidine kinase, alkaline DNase, catalase, acid phosphatase and lactic dehydrogenase has been determined as a function of the division cycle. The method of synchronization developed here allows the continuous synchronous growth of mammalian cells in suspension culture. This system facilitates studies of cell cycle events since it provides a large amount of cellular material with minimal maintenance time required.
Description
Thesis: Sc. D., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1971
Includes bibliographical references (pages 211-215, 216a-216g).
Subjects
Nutrition and Food Science.
MIT Department
Massachusetts Institute of Technology. Department of Nutrition and Food Science
Terms of Use
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
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