Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays
Name
life-11-00179.pdf
Size
570.14 KB
Format
Unknown
Checksum (MD5)
f53a4cf6041c7b894b8ed7ae7db2635f
Author(s)
Beyer, Sebastian
Blocki, Anna
Cheung, Matthew Chung Yin
Wan, Zoe Ho Ying
Mehrjou, Babak
Kamm, Roger Dale
Date Issued
February 2021
Journal
Life
Publisher
Multidisciplinary Digital Publishing Institute
Citation
Life 11 (3): 179 (2021)
Version
Final published version
Abstract
The endothelial glycocalyx forms the inner-most lining of human microvasculature. It ensures the physiological function of blood vessels and plays a crucial role in the occurrence and progression of microvascular diseases. The present communication aims to highlight the usefulness of high-resolution imaging of lectin (Bandeiraea Simplicifolia) stained endothelial glycocalyx in 3-dimensional microfluidic cell cultures. The microfluidic system allowed visualizing cancer cell extravasation, which is a key event in metastasis formation in cancer pathologies. In brief, microvascular networks were created through spontaneous vasculogenesis. This occurred from 3 dimensional (3D) suspensions of human umbilical vein endothelial cells (HUVECs) in hydrogels confined within microfluidic devices. Extravasation of MDA-MB-231 breast cancer cells from perfusable endothelial lumens was observed with confocal imaging of lectin-stained microvascular networks. The present work provides guidance towards optimizing the methodology used to elucidate the role of the endothelial glycocalyx during cancer cell extravasation. In particular, a high-resolution view of the endothelial glycocalyx at the site of extravasation is presented. The occurrence of glycocalyx defects is well aligned with the contemporary notion in the field that glycocalyx shedding precedes cancer cell extravasation.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/life11030179
