Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium
Name
s42003-022-03050-3.pdf
Description
Published version
Size
4.93 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Vickovic, Sanja
Schapiro, Denis
Carlberg, Konstantin
Lötstedt, Britta
Larsson, Ludvig
Hildebrandt, Franziska
Korotkova, Marina
Hensvold, Aase H
Catrina, Anca I
Sorger, Peter K
Date Issued
2022
Journal
Communications Biology
Publisher
Springer Science and Business Media LLC
Citation
Vickovic, Sanja, Schapiro, Denis, Carlberg, Konstantin, Lötstedt, Britta, Larsson, Ludvig et al. 2022. "Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium." Communications Biology, 5 (1).
Version
Final published version
Abstract
AbstractThe inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/S42003-022-03050-3
