The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing
Name
Brar-2009-The Multiple Roles o.pdf
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1.11 MB
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Author(s)
Brar, Gloria A.
Ee, Ly-Sha
Hochwagen, Andreas
Amon, Angelika B
Date Issued
February 2009
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Brar, Gloria A. et al. “The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing.” Mol. Biol. Cell 20.3 (2009): 1030-1047. ©2009 by The American Society for Cell Biology.
Version
Final published version
Abstract
Sister chromatid cohesion, mediated by cohesin complexes, is laid down during DNA replication and is essential for the accurate segregation of chromosomes. Previous studies indicated that, in addition to their cohesion function, cohesins are essential for completion of recombination, pairing, meiotic chromosome axis formation, and assembly of the synaptonemal complex (SC). Using mutants in the cohesin subunit Rec8, in which phosphorylated residues were mutated to alanines, we show that cohesin phosphorylation is not only important for cohesin removal, but that cohesin's meiotic prophase functions are distinct from each other. We find pairing and SC formation to be dependent on Rec8, but independent of the presence of a sister chromatid and hence sister chromatid cohesion. We identified mutations in REC8 that differentially affect Rec8's cohesion, pairing, recombination, chromosome axis and SC assembly function. These findings define Rec8 as a key determinant of meiotic chromosome morphogenesis and a central player in multiple meiotic events.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1091/mbc.E08-06-0637
