Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
Name
elife-78779-v1.pdf
Description
Published version
Size
6.79 MB
Format
Adobe PDF
Checksum (MD5)
15aa8e543c0274c3989d8749f1237fbd
Author(s)
Camuglia, Jaclyn
Chanet, Soline
Martin, Adam C
Date Issued
2022
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Camuglia, Jaclyn, Chanet, Soline and Martin, Adam C. 2022. "Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation." eLife, 11.
Version
Final published version
Abstract
Spindle orientation is often achieved by a complex of Partner of Inscuteable (Pins)/LGN, Mushroom Body Defect (Mud)/Nuclear Mitotic Apparatus (NuMa), Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early Drosophila embryo. We found that neither planar cell polarity pathways nor planar polarized myosin localization determined division orientation; instead, our findings strongly suggest that Pins planar polarity and force generated from mesoderm invagination are important. Disrupting Pins polarity via overexpression of a myristoylated version of Pins caused randomized division angles. We found that disrupting forces through chemical inhibitors, depletion of an adherens junction protein, or blocking mesoderm invagination disrupted Pins planar polarity and spindle orientation. Furthermore, directional ablations that separated mesoderm from mitotic domains disrupted spindle orientation, suggesting that forces transmitted from mesoderm to mitotic domains can polarize Pins and orient division during gastrulation. To our knowledge, this is the first in vivo example where mechanical force has been shown to polarize Pins to mediate division orientation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.7554/ELIFE.78779
