Endotoxin-Free Preparation of Graphene Oxide and Graphene-Based Materials for Biological Applications
Name
nihms-983033.pdf
Description
Accepted version
Size
940.55 KB
Format
Adobe PDF
Checksum (MD5)
5953ecf5f1a4d5c212298d2ed96a4ca2
Author(s)
Parviz, Dorsa
Strano, Michael S.
Date Issued
October 2018
Journal
Current Protocols
Publisher
Wiley
Citation
Parviz, Dorsa and Michael Strano. "Endotoxin-Free Preparation of Graphene Oxide and Graphene-Based Materials for Biological Applications." Current Protocols 10, 4 (December 2018): e51 © 2018 John Wiley & Sons, Inc.
Version
Author's final manuscript
Abstract
Due to their two-dimensional structure and unique properties, graphene and its derivatives have been extensively studied for their potential applications in various fields ranging from electronics to composites. Particularly, their high surface area, electrical conductivity, mechanical strength, dispersability in aqueous phase, and possibility of surface modification make them promising candidates for biomedical applications including biosensing, drug delivery, tissue engineering, cell imaging, and therapeutics. The functioning of graphene nanosheets in these applications is dependent on their structure and properties, which are mainly determined by their preparation and processing methods. Exfoliation techniques are the most common methods for preparation of graphene nanosheets for biomedical applications due to their high yield and scalability. Further modification of these methods is necessary to produce biocompatible and toxin-free graphene that can be safely incorporated into biological media. Here, we describe protocols for chemical and mechanical exfoliation of graphite to produce endotoxin-free and highly stable graphene oxide and graphene dispersions. Additional protocols are provided for proper pre- and post-processing of nanosheets and endotoxin measurement techniques. © 2018 by John Wiley & Sons, Inc.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/cpch.51
