A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles
Name
d0an00938e.pdf
Description
Published version
Size
1.76 MB
Format
Adobe PDF
Checksum (MD5)
18591cf6d0b9199a219cef370d6e09ee
Author(s)
Juthani, Nidhi
Doyle, Patrick S
Date Issued
June 2020
Journal
The Analyst
Publisher
Royal Society of Chemistry (RSC)
Citation
Juthani Nidhi and Patrick S. Doyle. "A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles." Analyst 2020 (June 2020)
© 2020 Royal Society of Chemistry
Version
Final published version
Abstract
We report a platform utilizing a reporter enzyme, which produces a chromogenic indigo precipitate that preferentially localizes within a hydrogel microparticle. The 3D network of the hydrogel maintains the rapid target binding kinetics found in solution, while multiplexed target detection is achieved through shape-encoding of the particles. Moreover, the precipitate-laden hydrogels can be imaged with a simple phone camera setup. We used this system to detect microRNA (miRNA) down to 0.22 fmol. We then showed the compatibility of this system with real samples by performing multiplexed miRNA measurements from total RNA from matched colon cancer and normal adjacent tissue.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1039/d0an00938e
