Multi-Stage Tuberculosis Subunit Vaccine Candidate LT69 Provides High Protection against Mycobacterium tuberculosis Infection in Mice
Name
Niu-2015-Multi-Stage Tubercul.pdf
Size
802.71 KB
Format
Adobe PDF
Checksum (MD5)
ea1b86a91086a4888be98230cab368a8
Author(s)
Niu, Hongxia
Peng, Jinxiu
Bai, Chunxiang
Liu, Xun
Hu, Lina
Luo, Yanping
Wang, Bingxiang
Zhang, Ying
Chen, Jianzhu
Yu, Hongjuan
Date Issued
June 2015
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Niu, Hongxia, Jinxiu Peng, Chunxiang Bai, Xun Liu, Lina Hu, Yanping Luo, Bingxiang Wang, et al. “Multi-Stage Tuberculosis Subunit Vaccine Candidate LT69 Provides High Protection Against Mycobacterium Tuberculosis Infection in Mice.” Edited by Ann Rawkins. PLoS ONE 10, no. 6 (June 22, 2015): e0130641.
Version
Final published version
Abstract
Effective tuberculosis (TB) vaccine should target tubercle bacilli with various metabolic states and confer long-term protective immunity. In this study, we constructed a novel multi-stage TB subunit vaccine based on fusion protein ESAT6-Ag85B-MPT64(190-198)-Mtb8.4-HspX (LT69 for short) which combined early expressed antigens and latency-associated antigen. The fusion protein was mixed with an adjuvant being composed of N, N’-dimethyl-N, N’-dioctadecylammonium bromide (DDA) and polyriboinosinic polyribocytidylic acid (PolyI:C) to construct subunit vaccine, whose immunogenicity and protective ability were evaluated in C57BL/6 mice. The results showed that LT69 had strong immunogenicity and high protective effect against Mycobacterium tuberculosis (M. tuberculosis) H37Rv aerosol challenge. Low-dose (2 μg) of LT69 generated long-term immune memory responses and provided effective protection, which was even higher than traditional vaccine BCG did at 30 weeks post the last vaccination. In conclusion, multistage subunit vaccine LT69 showed high and long-term protection against M. tuberculosis infection in mice, whose effect could be enhanced by using a relative low dosage of antigen.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1371/journal.pone.0130641
