Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT
Name
Jaenisch_Transnuclear mice.pdf
Size
3.29 MB
Format
Adobe PDF
Checksum (MD5)
931e831d62d49a8051cbb6dd95cf61be
Author(s)
Suh, Heikyung
Jaenisch, Rudolf
Ploegh, Hidde
Kirak, Oktay
Frickel, Eva-Maria
Grotenbreg, Gijsbert M.
Alternative Title
Transnuclear Mice with Predefined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT
Date Issued
April 2010
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Kirak, O., E.-M. Frickel, G. M. Grotenbreg, H. Suh, R. Jaenisch, and H. L. Ploegh. “Transnuclear Mice with Predefined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT.” Science 328, no. 5975 (April 8, 2010): 243-248.
Version
Author's final manuscript
Abstract
Mice that are transgenic for rearranged antigen-specific T cell receptors (TCRs) are essential tools to study T cell development and function. Such TCRs are usually isolated from the relevant T cells after long-term culture, often after repeated antigen stimulation, which unavoidably skews the T cell population used. Random genomic integration of the TCR α and β chain and expression from nonendogenous promoters represent additional drawbacks of transgenics. Using epigenetic reprogramming via somatic cell nuclear transfer, we demonstrated that T cells with predefined specificities against Toxoplasma gondii can be used to generate mouse models that express the TCR from their endogenous loci, without experimentally introduced genetic modification. The relative ease and speed with which such transnuclear models can be obtained holds promise for the construction of other disease models.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/science.1178590
