Excised linear introns regulate growth in yeast
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nihms-1009294.pdf
Description
Accepted version
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2.26 MB
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Author(s)
Morgan, Jeffrey T. (Jeffrey Thomas)
Bartel, David
Fink, Gerald R
Date Issued
January 2019
Journal
Nature
Publisher
Springer Nature
Citation
Morgan1, Jeffrey T., Fink, Gerald R. and Bartel, David P. "Excised linear introns regulate growth in yeast." Nature 565, 7741 (January 2019): 606–611 © 2019, Springer Nature Limited.
Version
Author's final manuscript
Abstract
Spliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid debranching and degradation. Here we describe 34 excised introns in Saccharomyces cerevisiae that—despite being rapidly degraded in log-phase growth—accumulate as linear RNAs under either saturated-growth conditions or other stresses that cause prolonged inhibition of TORC1, which is a key integrator of growth signalling. Introns that become stabilized remain associated with components of the spliceosome and differ from other spliceosomal introns in having a short distance between their lariat branch point and 3′ splice site, which is necessary and sufficient for their stabilization. Deletion of these unusual introns is disadvantageous in saturated conditions and causes aberrantly high growth rates in yeast that are chronically challenged with the TORC1 inhibitor rapamycin. The reintroduction of native or engineered stable introns suppresses this aberrant rapamycin response. Thus, excised introns function within the TOR growth-signalling network of S. cerevisiae and, more generally, excised spliceosomal introns can have biological functions. Keywords: Non-coding RNAs; RNA; TOR signalling; Transcriptomics
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41586-018-0828-1
