Versatile and on-demand biologics co-production in yeast
Name
s41467-017-02587-w.pdf
Size
1.78 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Cao, Jicong
Perez-Pinera, Pablo
Lowenhaupt, Ky
Wu, Ming-Ru
Purcell, Oliver
de la Fuente-Nunez, Cesar
Lu, Timothy K.
de la Fuente Nunez, Cesar
Lu, Timothy K
Date Issued
January 2018
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Cao, Jicong et al. “Versatile and on-Demand Biologics Co-Production in Yeast.” Nature Communications 9, 1 (January 2018): 77 © 2017 The Author(s)
Version
Final published version
Abstract
Current limitations to on-demand drug manufacturing can be addressed by technologies that streamline manufacturing processes. Combining the production of two or more drugs into a single batch could not only be useful for research, clinical studies, and urgent therapies but also effective when combination therapies are needed or where resources are scarce. Here we propose strategies to concurrently produce multiple biologics from yeast in single batches by multiplexing strain development, cell culture, separation, and purification. We demonstrate proof-of-concept for three biologics co-production strategies: (i) inducible expression of multiple biologics and control over the ratio between biologic drugs produced together; (ii) consolidated bioprocessing; and (iii) co-expression and co-purification of a mixture of two monoclonal antibodies. We then use these basic strategies to produce drug mixtures as well as to separate drugs. These strategies offer a diverse array of options for on-demand, flexible, low-cost, and decentralized biomanufacturing applications without the need for specialized equipment.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.1038/s41467-017-02587-w
