Proliferation and Tumorigenesis of a Murine Sarcoma Cell Line in the Absence of DICER1
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Ravi-2012-Proliferation and Tu.pdf
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Author(s)
Ravi, Arvind
Gurtan, Allan M.
Kumar, Madhu Sudhan
Chin, Christine
Lu, Victoria
Sharp, Phillip A.
Bhutkar, Arjun (AJ)
Lees, Jacqueline
Jacks, Tyler E.
Sharp, Phillip A.
Date Issued
June 2012
Journal
Cancer Cell
Publisher
Elsevier
Citation
Ravi, Arvind, Allan M. Gurtan, Madhu S. Kumar, Arjun Bhutkar, Christine Chin, Victoria Lu, Jacqueline A. Lees, Tyler Jacks, and Phillip A. Sharp. “Proliferation and Tumorigenesis of a Murine Sarcoma Cell Line in the Absence of DICER1.” Cancer Cell 21, no. 6 (June 2012): 848–855. © 2012 Elsevier Inc.
Version
Final published version
Abstract
MicroRNAs are a class of short ~22 nucleotide RNAs predicted to regulate nearly half of all protein coding genes, including many involved in basal cellular processes and organismal development. Although a global reduction in miRNAs is commonly observed in various human tumors, complete loss has not been documented, suggesting an essential function for miRNAs in tumorigenesis. Here we present the finding that transformed or immortalized Dicer1 null somatic cells can be isolated readily in vitro, maintain the characteristics of DICER1-expressing controls and remain stably proliferative. Furthermore, Dicer1 null cells from a sarcoma cell line, though depleted of miRNAs, are competent for tumor formation. Hence, miRNA levels in cancer may be maintained in vivo by a complex stabilizing selection in the intratumoral environment.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.ccr.2012.04.037
