Electrostatic Conjugation of Nanoparticle Surfaces with Functional Peptide Motifs
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nihms-1668334.pdf
Description
Accepted version
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1.7 MB
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Author(s)
Boehnke, Natalie
Dolph, Kate J
Juarez, Valeria M
Lanoha, Julia M
Hammond, Paula T
Date Issued
2020
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society (ACS)
Citation
Boehnke, Natalie, Dolph, Kate J, Juarez, Valeria M, Lanoha, Julia M and Hammond, Paula T. 2020. "Electrostatic Conjugation of Nanoparticle Surfaces with Functional Peptide Motifs." Bioconjugate Chemistry, 31 (9).
Version
Author's final manuscript
Abstract
Copyright © 2020 American Chemical Society. We report the surface functionalization of anionic layer by layer nanoparticles (LbL NPs) with cationic tumor-penetrating peptides (TPPs) via electrostatic adsorption while retaining particle stability and charge characteristics. This strategy eliminates the need for structural modifications of the peptide and enables facile functionalization of surface chemistries difficult to modify or inaccessible via covalent conjugation strategies. We show that both carboxylated and sulfated LbL NPs are able to accommodate linear and cyclic TPPs and used fluorescence-based detection assays to quantify peptide loading per NP. We also demonstrate that TPP activity is retained upon adsorption, implying sufficient numbers of peptides take on the appropriate surface orientation, enabling efficient uptake of functionalized NPs in vitro, as characterized via flow cytometry and deconvolution microscopy. Overall, we believe that this strategy will serve as a broadly applicable approach to impart electrostatically assembled NPs with bioactive peptide motifs.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1021/ACS.BIOCONJCHEM.0C00384
