Genes with internal repeats require the THO complex for transcription
Name
71225146-MIT.pdf
Description
Full printable version
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9.97 MB
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Checksum (MD5)
a572682cf1f22d267d5b4122cfab7d54
Author(s)
Voynov, Vladimir
Advisor(s)
Gerald R. Fink.
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
A genome-wide screen for promoter-independent regulators of the Saccharomyces cerevisiae adhesin FLOII identified 37 novel regulators. Among the mutants with the strongest phenotype were the deletions of genes encoding subunits of the evolutionarily conserved THO complex. The THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO I and other yeast genes that have long internal tandem repeats. FLO I transcription elongation in Tho- mutants is hindered in the region of the tandem repeats, resulting in a loss of function. Moreover, the repeats become genetically unstable in Tho- mutants. A FLO I gene without the tandem repeats is transcribed equally well in Tho+ or Tho- strains. The Tho- defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription.
Description
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, June 2006.
"May 2006."
Includes bibliographical references.
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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