Mechanisms for the Intracellular Manipulation of Organelles by Conventional Electroporation
Name
Esser-2010-Mechanisms for the I.pdf
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1.84 MB
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Author(s)
Esser, Axel Thomas
Smith, Kyle C.
Gowrishankar, Thiruvallur R.
Vasilkoski, Zlatko
Weaver, James C.
Date Issued
June 2010
Journal
Biophysical Journal
Publisher
Elsevier B.V.
Citation
Esser, Axel T., Kyle C. Smith, T.R. Gowrishankar, Zlatko Vasilkoski, and James C. Weaver. “Mechanisms for the Intracellular Manipulation of Organelles by Conventional Electroporation.” Biophysical Journal 98, no. 11 (June 2010): 2506–2514. © 2010 Biophysical Society.
Version
Final published version
Abstract
Conventional electroporation (EP) changes both the conductance and molecular permeability of the plasma membrane (PM) of cells and is a standard method for delivering both biologically active and probe molecules of a wide range of sizes into cells. However, the underlying mechanisms at the molecular and cellular levels remain controversial. Here we introduce a mathematical cell model that contains representative organelles (nucleus, endoplasmic reticulum, mitochondria) and includes a dynamic EP model, which describes formation, expansion, contraction, and destruction for the plasma and all organelle membranes. We show that conventional EP provides transient electrical pathways into the cell, sufficient to create significant intracellular fields. This emerging intracellular electrical field is a secondary effect due to EP and can cause transmembrane voltages at the organelles, which are large enough and long enough to gate organelle channels, and even sufficient, at some field strengths, for the poration of organelle membranes. This suggests an alternative to nanosecond pulsed electric fields for intracellular manipulations.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2010.02.035
