Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell
Name
Fink_Rapid capture.pdf
Description
Accepted version
Size
942.15 KB
Format
Adobe PDF
Checksum (MD5)
7dd644c088d0e905c6028db830f51aa2
Author(s)
Chen, Guan-Yu
Li, Zeyang
Duarte, Joao N.
Esteban, Alexandre
Cheloha, Ross W.
Theile, Christopher S
Ploegh, Hidde
Fink, Gerald R
Date Issued
March 2017
Journal
Biosensors and Bioelectronics
Publisher
Elsevier BV
Citation
Chen, Guan-Yu, Zeyang Li, Joao N. Duarte et al. "Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell" Biosensors and Bioelectronics 89,2 (March 2017):789-794. © 2016 Elsevier B.V.
Version
Author's final manuscript
Abstract
Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes. Keywords: Single domain antibody, Graphene oxide, Sortase, Enzymatic labeling, Cell detection, Leukocytes
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.BIOS.2016.10.015
