Gas Vesicles across Kingdoms: A Comparative Solid-State Nuclear Magnetic Resonance Study
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Griffin_Gas vesicles.pdf
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2.47 MB
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Author(s)
Daviso, Eugenio
Belenky, Marina
Herzfeld, Judith
Griffin, Robert Guy
Date Issued
August 2013
Journal
Journal of Molecular Microbiology and Biotechnology
Publisher
S. Karger AG
Citation
Daviso, Eugenio, Marina Belenky, Robert G. Griffin, and Judith Herzfeld. “Gas Vesicles Across Kingdoms: A Comparative Solid-State Nuclear Magnetic Resonance Study.” Journal of Molecular Microbiology and Biotechnology 23, no. 4–5 (2013): 281–289.
Version
Author's final manuscript
Abstract
The buoyancy organelles of aquatic microorganisms have to meet stringent specifications: allowing gases to equilibrate freely across the proteinaceous shell, preventing the condensation of water vapor inside the hollow cavity and resisting collapse under hydrostatic pressures that vary with column depth. These properties are provided by the 7- to 8-kDa gas vesicle protein A (GvpA), repeats of which form all but small, specialized portions of the shell. Magic angle spinning nuclear magnetic resonance is uniquely capable of providing high-resolution information on the fold and assembly of GvpA. Here we compare results for the gas vesicles of the haloarchaea Halobacterium salinarum with those obtained previously for the cyanobacterium Anabaena flos-aquae. The data suggest that the two organisms follow similar strategies for avoiding water condensation. On the other hand, in its relatively shallow habitat, H. salinarum is able to avoid collapse with a less costly GvpA fold than is adopted by A. flos-aquae.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1159/000351340
