Muscle functions as a connective tissue and source of extracellular matrix in planarians
Name
s41467-019-09539-6.pdf
Description
Published version
Size
6.97 MB
Format
Adobe PDF
Checksum (MD5)
da1f1053e39e8c7caed31c4001fac3a6
Author(s)
Cote, Lauren Esther
Simental, Eric
Reddien, Peter
Date Issued
April 2019
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Cote, Lauren E. et al. "Muscle functions as a connective tissue and source of extracellular matrix in planarians." Nature Communications 10 (April 2019): 1592 © 2019 The Author(s)
Version
Final published version
Abstract
Regeneration and tissue turnover require new cell production and positional information. Planarians are flatworms capable of regenerating all body parts using a population of stem cells called neoblasts. The positional information required for tissue patterning is primarily harbored by muscle cells, which also control body contraction. Here we produce an in silico planarian matrisome and use recent whole-animal single-cell-transcriptome data to determine that muscle is a major source of extracellular matrix (ECM). No other ECM-secreting, fibroblast-like cell type was detected. Instead, muscle cells express core ECM components, including all 19 collagen-encoding genes. Inhibition of muscle-expressed hemicentin-1 (hmcn-1), which encodes a highly conserved ECM glycoprotein, results in ectopic peripheral localization of cells, including neoblasts, outside of the muscle layer. ECM secretion and hmcn-1-dependent maintenance of tissue separation indicate that muscle functions as a planarian connective tissue, raising the possibility of broad roles for connective tissue in adult positional information.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-019-09539-6
