Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates
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Author(s)
Acker, Leah Christine
Pino, Erica N.
Boyden, Edward
Desimone, Robert
Date Issued
October 2017
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Citation
Acker, Leah C et al. “Large Volume, Behaviorally-Relevant Illumination for Optogenetics in Non-Human Primates.” Journal of Visualized Experiments 128 (October 2017) © 2017 Journal of Visualized Experiments
Version
Final published version
Abstract
This protocol describes a large-volume illuminator, which was developed for optogenetic manipulations in the non-human primate brain. The illuminator is a modified plastic optical fiber with etched tip, such that the light emitting surface area is > 100x that of a conventional fiber. In addition to describing the construction of the large-volume illuminator, this protocol details the quality-control calibration used to ensure even light distribution. Further, this protocol describes techniques for inserting and removing the large volume illuminator. Both superficial and deep structures may be illuminated. This large volume illuminator does not need to be physically coupled to an electrode, and because the illuminator is made of plastic, not glass, it will simply bend in circumstances when traditional optical fibers would shatter. Because this illuminator delivers light over behaviorally-relevant tissue volumes (≈ 10 mm 3 ) with no greater penetration damage than a conventional optical fiber, it facilitates behavioral studies using optogenetics in non-human primates.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.3791/56330
