Highly Parallel Profiling of Cas9 Variant Specificity
Name
nihms-1577774.pdf
Description
Accepted version
Size
1.25 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Schmid-Burgk, JL
Gao, L
Li, D
Gardner, Z
Strecker, J
Lash, B
Zhang, F
Date Issued
May 21, 2020
Journal
Molecular Cell
Publisher
Elsevier BV
Citation
Schmid-Burgk, JL, Gao, L, Li, D, Gardner, Z, Strecker, J et al. 2020. "Highly Parallel Profiling of Cas9 Variant Specificity." Molecular Cell, 78 (4).
Version
Author's final manuscript
Abstract
© 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off between specificity and activity and identifying LZ3 Cas9, a variant with a unique +1 insertion profile.
MIT Department
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
Howard Hughes Medical Institute
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.molcel.2020.02.023
