Cas9 Functionally Opens Chromatin
Name
Barkal-2016-Cas9 Functionally Op.pdf
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483.67 KB
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Author(s)
Barkal, Amira A.
Srinivasan, Sharanya
Gifford, David K.
Sherwood, Richard I.
Hashimoto, Tatsunori Benjamin
Date Issued
March 2016
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Barkal, Amira A., Sharanya Srinivasan, Tatsunori Hashimoto, David K. Gifford, and Richard I. Sherwood. “Cas9 Functionally Opens Chromatin.” Edited by Hodaka Fujii. PLOS ONE 11, no. 3 (March 31, 2016): e0152683.
Version
Final published version
Abstract
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0152683
