Short Cationic Peptide Derived from Archaea with Dual Antibacterial Properties and Anti-Infective Potential
Name
acsinfecdis.9b00073.pdf
Description
Accepted version
Size
1.58 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Cândido, Elizabete S
Cardoso, Marlon H
Chan, Lai Y
Torres, Marcelo DT
Oshiro, Karen GN
Porto, William F
Ribeiro, Suzana M
Haney, Evan F
Hancock, Robert EW
Lu, Timothy K
Date Issued
2019
Journal
ACS Infectious Diseases
Publisher
American Chemical Society (ACS)
Version
Author's final manuscript
Abstract
Copyright © 2019 American Chemical Society. Bacterial biofilms and associated infections represent one of the biggest challenges in the clinic, and as an alternative to counter bacterial infections, antimicrobial peptides have attracted great attention in the past decade. Here, ten short cationic antimicrobial peptides were generated through a sliding-window strategy on the basis of the 19-amino acid residue peptide, derived from a Pyrobaculum aerophilum ribosomal protein. PaDBS1R6F10 exhibited anti-infective potential as it decreased the bacterial burden in murine Pseudomonas aeruginosa cutaneous infections by more than 1000-fold. Adverse cytotoxic and hemolytic effects were not detected against mammalian cells. The peptide demonstrated structural plasticity in terms of its secondary structure in the different environments tested. PaDBS1R6F10 represents a promising antimicrobial agent against bacteria infections, without harming human cells.
MIT Department
Massachusetts Institute of Technology. Synthetic Biology Center
Massachusetts Institute of Technology. Center for Microbiome Informatics and Therapeutics
Massachusetts Institute of Technology. Research Laboratory of Electronics
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
10.1021/acsinfecdis.9b00073
https://doi.org/10.1021/acsinfecdis.9b00073
