Simple filter microchip for rapid separation of plasma and viruses from whole blood
Name
Wang-2012-Simple filter microc.pdf
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Author(s)
Wang, ShuQi
Sarenac, Dusan
Chen, Michael H.
Huang, Shih-Han
Giguel, Francoise F.
Kuritzkes, Daniel R.
Demirci, Utkan
Date Issued
September 2012
Journal
International Journal of Nanomedicine
Publisher
Dove Medical Press
Citation
Wang, ShuQi, Dusan Sarenac, Michael H. Chen, Shih-Han Huang, Francoise F. Giguel, Daniel R. Kuritzkes, and Utkan Demirci. “Simple Filter Microchip for Rapid Separation of Plasma and Viruses from Whole Blood.” International Journal of Nanomedicine (September 2012): 5019.
Version
Final published version
Abstract
Sample preparation is a significant challenge for detection and sensing technologies, since the presence of blood cells can interfere with the accuracy and reliability of virus detection at the nanoscale for point-of-care testing. To the best of our knowledge, there is not an existing on-chip virus isolation technology that does not use complex fluidic pumps. Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump. We demonstrated that viruses (eg, HIV) can be separated on a filter-based chip (2-µm pore size) from HIV-spiked whole blood at high recovery efficiencies of 89.9% ± 5.0%, 80.5% ± 4.3%, and 78.2% ± 3.8%, for viral loads of 1000, 10,000 and 100,000 copies/mL, respectively. Meanwhile, 81.7% ± 6.7% of red blood cells and 89.5% ± 2.4% of white blood cells were retained on 2 µm pore–sized filter microchips. We also tested these filter microchips with seven HIV-infected patient samples and observed recovery efficiencies ranging from 73.1% ± 8.3% to 82.5% ± 4.1%. These results are first steps towards developing disposable point-of-care diagnostics and monitoring devices for resource-constrained settings, as well as hospital and primary care settings.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution-Noncommercial
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DOI of Published Version
https://doi.org/10.2147/ijn.s32579
