A vector-free microfluidic platform for intracellular delivery
Name
Shareia-2013-A vector-free microfluidic platform for intracellular delivery.pdf
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914.7 KB
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Checksum (MD5)
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Author(s)
Sharei, Armon Reza
Zoldan, Janeta
Sim, Woo Young
Cho, Nahyun
Jackson, Emily L.
Mao, Shirley
Schneider, Sabine
Kim, Kwang-Soo
Han, Min-Joon
Lytton-Jean, Abigail K. R.
Date Issued
January 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Sharei, A., J. Zoldan, A. Adamo, W. Y. Sim, N. Cho, E. Jackson, S. Mao, et al. “A vector-free microfluidic platform for intracellular delivery.” Proceedings of the National Academy of Sciences 110, no. 6 (February 5, 2013): 2082-2087.
Version
Final published version
Abstract
Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a constriction 30–80% smaller than the cell diameter. The resulting controlled application of compression and shear forces results in the formation of transient holes that enable the diffusion of material from the surrounding buffer into the cytosol. The method has demonstrated the ability to deliver a range of material, such as carbon nanotubes, proteins, and siRNA, to 11 cell types, including embryonic stem cells and immune cells. When used for the delivery of transcription factors, the microfluidic devices produced a 10-fold improvement in colony formation relative to electroporation and cell-penetrating peptides. Indeed, its ability to deliver structurally diverse materials and its applicability to difficult-to-transfect primary cells indicate that this method could potentially enable many research and clinical applications.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Spectroscopy Laboratory
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1218705110
