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A comparison of microfluidic methods for high-throughput cell deformability measurements
Name
nihms-1579163.pdf
Description
Accepted version
Size
1.38 MB
Format
Adobe PDF
Checksum (MD5)
cf3541c433f30fc31b3edcda6e4dd5e0
Author(s)
Urbanska, Marta
Muñoz, Hector E
Shaw Bagnall, Josephine
Otto, Oliver
Manalis, Scott R
Di Carlo, Dino
Guck, Jochen
Date Issued
2020
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Version
Author's final manuscript
Abstract
The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we present a standardized cross-laboratory study comparing three microfluidics-based approaches for measuring cell mechanical phenotype: constriction-based deformability cytometry (cDC), shear flow deformability cytometry (sDC) and extensional flow deformability cytometry (xDC). All three methods detect cell deformability changes induced by exposure to altered osmolarity. However, a dose-dependent deformability increase upon latrunculin B-induced actin disassembly was detected only with cDC and sDC, which suggests that when exposing cells to the higher strain rate imposed by xDC, cellular components other than the actin cytoskeleton dominate the response. The direct comparison presented here furthers our understanding of the applicability of the different deformability cytometry methods and provides context for the interpretation of deformability measurements performed using different platforms.
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DOI of Published Version
10.1038/S41592-020-0818-8
