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Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions
Name
nihms969445.pdf
Description
Accepted version
Size
1.31 MB
Format
Adobe PDF
Checksum (MD5)
9c78a46015b7915b65c024529126f735
Author(s)
Kim, Jae Jung
Reátegui, Eduardo
Hopke, Alex
Jalali, Fatemeh
Roushan, Maedeh
Doyle, Patrick S
Irimia, Daniel
Date Issued
2018
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Version
Author's final manuscript
Abstract
© 2018 The Royal Society of Chemistry. Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug.
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.1039/C8LC00228B
