Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity
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Langer_Self-assembled gold.pdf
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1.18 MB
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Author(s)
Mu, C. Jenny
LaVan, David A.
Langer, Robert
Zetter, Bruce R.
Date Issued
February 2010
Journal
ACS Nano
Publisher
American Chemical Society
Citation
Mu, C. Jenny, David A. LaVan, Robert S. Langer, and Bruce R. Zetter. "Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity In Vivo." ACS Nano 4, no. 3 (March 23, 2010): 1511-1520.
Version
Author's final manuscript
Abstract
Target-activatable fluorogenic probes based on gold nanoparticles (AuNPs) functionalized with self-assembled heterogeneous monolayers of dye-labeled peptides and poly(ethylene glycol) have been developed to visualize proteolytic activity in vivo. A one-step synthesis strategy that allows simple generation of surface-defined AuNP probe libraries is presented as a means of tailoring and evaluating probe characteristics for maximal fluorescence enhancement after protease activation. Optimal AuNP probes targeted to trypsin and urokinase-type plasminogen activator required the incorporation of a dark quencher to achieve 5- to 8-fold signal amplification. These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/nn9017334
