Stepwise nucleosome translocation by RSC remodeling complexes
Name
Harada-2016-Stepwise nucleosome.pdf
Size
2.16 MB
Format
Adobe PDF
Checksum (MD5)
589169cc1629157055885e64afb2fc03
Author(s)
Deindl, Sebastian
Chatterjee, Nilanjana
Bartholomew, Blaine
Zhuang, Xiaowei
Harada, Bryan T.
Hwang, William L.
Date Issued
February 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Harada, Bryan T, William L Hwang, Sebastian Deindl, Nilanjana Chatterjee, Blaine Bartholomew, and Xiaowei Zhuang. “Stepwise Nucleosome Translocation by RSC Remodeling Complexes.” eLife 5 (February 19, 2016).
Version
Final published version
Abstract
The SWI/SNF-family remodelers regulate chromatin structure by coupling the free energy from ATP hydrolysis to the repositioning and restructuring of nucleosomes, but how the ATPase activity of these enzymes drives the motion of DNA across the nucleosome remains unclear. Here, we used single-molecule FRET to monitor the remodeling of mononucleosomes by the yeast SWI/SNF remodeler, RSC. We observed that RSC primarily translocates DNA around the nucleosome without substantial displacement of the H2A-H2B dimer. At the sites where DNA enters and exits the nucleosome, the DNA moves largely along or near its canonical wrapping path. The translocation of DNA occurs in a stepwise manner, and at both sites where DNA enters and exits the nucleosome, the step size distributions exhibit a peak at approximately 1–2 bp. These results suggest that the movement of DNA across the nucleosome is likely coupled directly to DNA translocation by the ATPase at its binding site inside the nucleosome.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/eLife.10051
