Engineering thermostable affinity proteins for use in high-throughput immunoassay formats
Name
d3sd00041a.pdf
Description
Published version
Size
848.84 KB
Format
Adobe PDF
Checksum (MD5)
6fba3ce8a8c7c60ff6c8f1e1b07955ee
Author(s)
Jia, Huan
Moomen, Nazirulmubin Abdul
Leong, Jeanette
Kongsuphol, Patthara
Lim, Zhi Feng Sherman
Pui, Carmen Sze Min
Tan, Yuxuan
Sung, Ki-Joo
Sikes, Hadley D
Date Issued
2023
Journal
Sensors & Diagnostics
Publisher
Royal Society of Chemistry
Citation
Sens. Diagn., 2023,2, 627-631
Version
Final published version
Abstract
ELISA utilizes polystyrene materials for capture reagents. Natural absorption randomly orients the capture reagent, decreasing immobilization and target biomarker accessibility. A thermostable affinity protein rcSso7d can replace antibodies as a capture reagent in cellulose-based diagnostic tests. ‘RAFIASRRIRRP’ at the N-terminus of two repeating units of rcSso7d improved polystyrene immobilization for high-throughput ELISA.
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/d3sd00041a
