A high throughput approach for analysis of cell nuclear deformability at single cell level
Name
A high throughput.pdf
Size
1.45 MB
Format
Adobe PDF
Checksum (MD5)
cd31f714f824e07254663e8246084bc8
Author(s)
Ermis, Menekse
Chen, Pu
Demirci, Utkan
Hasirci, Vasif
Akkaynak Yellin, Derya
Date Issued
March 29, 2017
Journal
Scientific Reports
Citation
Ermis, Menekse et al. “A High Throughput Approach for Analysis of Cell Nuclear Deformability at Single Cell Level.” Scientific Reports 6.1 (2016): n. pag.
Version
Final published version
Abstract
Various physiological and pathological processes, such as cell differentiation, migration, attachment, and metastasis are highly dependent on nuclear elasticity. Nuclear morphology directly reflects the elasticity of the nucleus. We propose that quantification of changes in nuclear morphology on surfaces with defined topography will enable us to assess nuclear elasticity and deformability. Here, we used soft lithography techniques to produce 3 dimensional (3-D) cell culture substrates decorated with micron sized pillar structures of variable aspect ratios and dimensions to induce changes in cellular and nuclear morphology. We developed a high content image analysis algorithm to quantify changes in nuclear morphology at the single-cell level in response to physical cues from the 3-D culture substrate. We present that nuclear stiffness can be used as a physical parameter to evaluate cancer cells based on their lineage and in comparison to non-cancerous cells originating from the same tissue type. This methodology can be exploited for systematic study of mechanical characteristics of large cell populations complementing conventional tools such as atomic force microscopy and nanoindentation.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/srep36917
