Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes
Name
Shi-2014-Engineered red blood.pdf
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Author(s)
Shi, Jiahai
Kundrat, Lenka
Pishesha, Novalia
Bilate, Angelina M.
Theile, Christopher S.
Maruyama, Takeshi
Dougan, Stephanie K.
Ploegh, Hidde
Lodish, Harvey F.
Date Issued
June 2014
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Shi, J., L. Kundrat, N. Pishesha, A. Bilate, C. Theile, T. Maruyama, S. K. Dougan, H. L. Ploegh, and H. F. Lodish. “Engineered Red Blood Cells as Carriers for Systemic Delivery of a Wide Array of Functional Probes.” Proceedings of the National Academy of Sciences 111, no. 28 (June 30, 2014): 10131–10136.
Version
Final published version
Abstract
We developed modified RBCs to serve as carriers for systemic delivery of a wide array of payloads. These RBCs contain modified proteins on their plasma membrane, which can be labeled in a sortase-catalyzed reaction under native conditions without inflicting damage to the target membrane or cell. Sortase accommodates a wide range of natural and synthetic payloads that allow modification of RBCs with substituents that cannot be encoded genetically. As proof of principle, we demonstrate site-specific conjugation of biotin to in vitro-differentiated mouse erythroblasts as well as to mature mouse RBCs. Thus modified, RBCs remain in the bloodstream for up to 28 d. A single domain antibody attached enzymatically to RBCs enables them to bind specifically to target cells that express the antibody target. We extend these experiments to human RBCs and demonstrate efficient sortase-mediated labeling of in vitro-differentiated human reticulocytes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1073/pnas.1409861111
