Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytes
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Photopatterning of Hydrogel Scaffolds.pdf
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Author(s)
Neiman, Jaclyn A. Shepard
Raman, Ritu
Chan, Vincent
Rhoads, Mary G.
Velazquez, Jeremy J.
Bashir, Rashid
Hammond, Paula T.
Griffith, Linda G.
Raredon, Micha Sam Brickman
Dyer, Rachel Lee
Date Issued
April 2015
Journal
Biotechnology and Bioengineering
Publisher
Wiley Blackwell
Citation
Neiman, Jaclyn A. Shepard, Ritu Raman, Vincent Chan, Mary G. Rhoads, Micha Sam B. Raredon, Jeremy J. Velazquez, Rachel L. Dyer, Rashid Bashir, Paula T. Hammond, and Linda G. Griffith. “Photopatterning of Hydrogel Scaffolds Coupled to Filter Materials Using Stereolithography for Perfused 3D Culture of Hepatocytes.” Biotechnology and Bioengineering 112, no. 4 (February 23, 2015): 777–787.
Version
Author's final manuscript
Abstract
In vitro models that recapitulate the liver's structural and functional complexity could prolong hepatocellular viability and function to improve platforms for drug toxicity studies and understanding liver pathophysiology. Here, stereolithography (SLA) was employed to fabricate hydrogel scaffolds with open channels designed for post-seeding and perfused culture of primary hepatocytes that form 3D structures in a bioreactor. Photopolymerizable polyethylene glycol-based hydrogels were fabricated coupled to chemically activated, commercially available filters (polycarbonate and polyvinylidene fluoride) using a chemistry that permitted cell viability, and was robust enough to withstand perfused culture of up to 1 µL/s for at least 7 days. SLA energy dose, photoinitiator concentrations, and pretreatment conditions were screened to determine conditions that maximized cell viability and hydrogel bonding to the filter. Multiple open channel geometries were readily achieved, and included ellipses and rectangles. Rectangular open channels employed for subsequent studies had final dimensions on the order of 350 µm by 850 µm. Cell seeding densities and flow rates that promoted cell viability were determined. Perfused culture of primary hepatocytes in hydrogel scaffolds in the presence of soluble epidermal growth factor (EGF) prolonged the maintenance of albumin production throughout the 7-day culture relative to 2D controls. This technique of bonding hydrogel scaffolds can be employed to fabricate soft scaffolds for a number of bioreactor configurations and applications.
MIT Department
Massachusetts Institute of Technology. Center for Gynepathology Research
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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DOI of Published Version
https://doi.org/10.1002/bit.25494
