High-throughput functional genomics using CRISPR–Cas9
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Zhang_High-throughput.pdf
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1.4 MB
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Author(s)
Shalem, Ophir
Zhang, Feng
Sanjana, Neville
Date Issued
April 2015
Journal
Nature Reviews Genetics
Publisher
Nature Publishing Group
Citation
Shalem, Ophir, Neville E. Sanjana, and Feng Zhang. “High-Throughput Functional Genomics Using CRISPR–Cas9.” Nature Reviews Genetics 16, no. 5 (April 9, 2015): 299–311.
Version
Author's final manuscript
Abstract
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/nrg3899
